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    Structured Review

    New England Biolabs xbai sites
    Xbai Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6921 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xbai+site/XbaI/pm42844471-698-19-25
    Average 99 stars, based on 6921 article reviews
    xbai sites - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: ARF degradation defines a deeply conserved step in auxin response
    Article Snippet: .. Next, the plasmid pMpGWB100, carrying a hygromycin resistance casette, was digested with the restriction enzyme XbaI (Thermo Fisher Scientific), and the aforementioned MpARF2 promoter was ligated downstream of an XbaI site followed by the mNG coding sequence using NEBuilder HiFi DNA Assembly (New England Biolabs) . ..

    Article Title: Zebrafish Polymerase Theta and human Polymerase Theta: Orthologues with homologous function.
    Article Snippet: .. First, the polymerase domain of zebrafish Pol θ (residues 1832–2576) was amplified from the cDNA library with primers containing a 5’ KpnI site and a 3’ BamHI site: Pol θ RVS BamHI- TATACTGGATCCTTATATGTCCAGGTCTTGAAGGTTACC Pol θ FWD KpnI- ATTAGGTACCTCAACATCAGTGTTAGGCGCAC PLOS One | https://doi.org/10.1371/journal.pone.0321886 April 29, 2025 14 / 21 Second, the 6xHIS and SUMO sequences (HIS-SUMO) of POLQM1 were amplified off of the plasmid using primers containing a 5’ XbaI site and a 3’ KpnI site: HIS-SUMO RVS KpnI- ATTAGGTACCTCCCGTCTGCTGC HIS-SUMO FWD XbaI- TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAG PCRs reactions used Phusion High-Fidelity DNA Polymerase (NEB) following manufacturer’s instructions and were run for 30 cycles. .. PCR Products were gel isolated from a 1% TAE agarose gel using Freeze ‘N Squeeze DNA gel extraction columns (Bio-Rad), following manufacturer’s instructions.

    Article Title: ARF degradation defines a deeply conserved step in auxin response.
    Article Snippet: .. Next, the plasmid pMpGWB100, carrying a hygromycin resistance casette, was digested with the restriction enzyme XbaI (Thermo Fisher Scientific), and the aforementioned MpARF2 promoter was ligated downstream of an XbaI site followed by the mNG coding sequence using NEBuilder HiFi DNA Assembly (New England Biolabs)14. ..

    Article Title: Zebrafish Polymerase Theta and human Polymerase Theta: Orthologues with homologous function
    Article Snippet: .. First, the polymerase domain of zebrafish Pol θ (residues 1832–2576) was amplified from the cDNA library with primers containing a 5’ KpnI site and a 3’ BamHI site: Second, the 6xHIS and SUMO sequences (HIS-SUMO) of POLQM1 were amplified off of the plasmid using primers containing a 5’ XbaI site and a 3’ KpnI site: PCRs reactions used Phusion High-Fidelity DNA Polymerase (NEB) following manufacturer’s instructions and were run for 30 cycles. .. PCR Products were gel isolated from a 1% TAE agarose gel using Freeze ‘N Squeeze DNA gel extraction columns (Bio-Rad), following manufacturer’s instructions.

    Sequencing:

    Article Title: ARF degradation defines a deeply conserved step in auxin response
    Article Snippet: .. Next, the plasmid pMpGWB100, carrying a hygromycin resistance casette, was digested with the restriction enzyme XbaI (Thermo Fisher Scientific), and the aforementioned MpARF2 promoter was ligated downstream of an XbaI site followed by the mNG coding sequence using NEBuilder HiFi DNA Assembly (New England Biolabs) . ..

    Article Title: ARF degradation defines a deeply conserved step in auxin response.
    Article Snippet: .. Next, the plasmid pMpGWB100, carrying a hygromycin resistance casette, was digested with the restriction enzyme XbaI (Thermo Fisher Scientific), and the aforementioned MpARF2 promoter was ligated downstream of an XbaI site followed by the mNG coding sequence using NEBuilder HiFi DNA Assembly (New England Biolabs)14. ..

    Amplification:

    Article Title: Zebrafish Polymerase Theta and human Polymerase Theta: Orthologues with homologous function.
    Article Snippet: .. First, the polymerase domain of zebrafish Pol θ (residues 1832–2576) was amplified from the cDNA library with primers containing a 5’ KpnI site and a 3’ BamHI site: Pol θ RVS BamHI- TATACTGGATCCTTATATGTCCAGGTCTTGAAGGTTACC Pol θ FWD KpnI- ATTAGGTACCTCAACATCAGTGTTAGGCGCAC PLOS One | https://doi.org/10.1371/journal.pone.0321886 April 29, 2025 14 / 21 Second, the 6xHIS and SUMO sequences (HIS-SUMO) of POLQM1 were amplified off of the plasmid using primers containing a 5’ XbaI site and a 3’ KpnI site: HIS-SUMO RVS KpnI- ATTAGGTACCTCCCGTCTGCTGC HIS-SUMO FWD XbaI- TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAG PCRs reactions used Phusion High-Fidelity DNA Polymerase (NEB) following manufacturer’s instructions and were run for 30 cycles. .. PCR Products were gel isolated from a 1% TAE agarose gel using Freeze ‘N Squeeze DNA gel extraction columns (Bio-Rad), following manufacturer’s instructions.

    Article Title: Zebrafish Polymerase Theta and human Polymerase Theta: Orthologues with homologous function
    Article Snippet: .. First, the polymerase domain of zebrafish Pol θ (residues 1832–2576) was amplified from the cDNA library with primers containing a 5’ KpnI site and a 3’ BamHI site: Second, the 6xHIS and SUMO sequences (HIS-SUMO) of POLQM1 were amplified off of the plasmid using primers containing a 5’ XbaI site and a 3’ KpnI site: PCRs reactions used Phusion High-Fidelity DNA Polymerase (NEB) following manufacturer’s instructions and were run for 30 cycles. .. PCR Products were gel isolated from a 1% TAE agarose gel using Freeze ‘N Squeeze DNA gel extraction columns (Bio-Rad), following manufacturer’s instructions.

    cDNA Library Assay:

    Article Title: Zebrafish Polymerase Theta and human Polymerase Theta: Orthologues with homologous function.
    Article Snippet: .. First, the polymerase domain of zebrafish Pol θ (residues 1832–2576) was amplified from the cDNA library with primers containing a 5’ KpnI site and a 3’ BamHI site: Pol θ RVS BamHI- TATACTGGATCCTTATATGTCCAGGTCTTGAAGGTTACC Pol θ FWD KpnI- ATTAGGTACCTCAACATCAGTGTTAGGCGCAC PLOS One | https://doi.org/10.1371/journal.pone.0321886 April 29, 2025 14 / 21 Second, the 6xHIS and SUMO sequences (HIS-SUMO) of POLQM1 were amplified off of the plasmid using primers containing a 5’ XbaI site and a 3’ KpnI site: HIS-SUMO RVS KpnI- ATTAGGTACCTCCCGTCTGCTGC HIS-SUMO FWD XbaI- TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAG PCRs reactions used Phusion High-Fidelity DNA Polymerase (NEB) following manufacturer’s instructions and were run for 30 cycles. .. PCR Products were gel isolated from a 1% TAE agarose gel using Freeze ‘N Squeeze DNA gel extraction columns (Bio-Rad), following manufacturer’s instructions.

    Article Title: Zebrafish Polymerase Theta and human Polymerase Theta: Orthologues with homologous function
    Article Snippet: .. First, the polymerase domain of zebrafish Pol θ (residues 1832–2576) was amplified from the cDNA library with primers containing a 5’ KpnI site and a 3’ BamHI site: Second, the 6xHIS and SUMO sequences (HIS-SUMO) of POLQM1 were amplified off of the plasmid using primers containing a 5’ XbaI site and a 3’ KpnI site: PCRs reactions used Phusion High-Fidelity DNA Polymerase (NEB) following manufacturer’s instructions and were run for 30 cycles. .. PCR Products were gel isolated from a 1% TAE agarose gel using Freeze ‘N Squeeze DNA gel extraction columns (Bio-Rad), following manufacturer’s instructions.

    Transformation Assay:

    Article Title: Supporting Information for FlaG competes with FliS-flagellin complexes for access to FlhA in the flagellar T3SS to control Campylobacter jejuni filament length
    Article Snippet: .. This fragment was inserted into the XbaI site of pGP704sacB28 by Gibson Assembly (New England Biolabs) and the resulting product was transformed into DH5 pir to create pDRH5543. ..

    Ligation:

    Article Title: Chromatin modification abnormalities by CHD7 and KMT2C loss promote medulloblastoma progression.
    Article Snippet: .. Subsequently, the U6-sgGOI cassette was inserted into the XbaI site of pX330-sgPtch1 by ligation (NEB, M2200) in the presence of XbaI and BbsI (Thermo, FD0684 and FD1014). ..

    Clone Assay:

    Article Title: Method of producing a tripeptide gamma-glu-val-gly using enterobacteriaceae
    Article Snippet: .. The gshB fragment was cloned at XbaI site of pUC19 (New England Biolabs) using in-fusion technique (In-Fusion HD cloning Kit, Clontech). ..

    Cloning:

    Article Title: Method of producing a tripeptide gamma-glu-val-gly using enterobacteriaceae
    Article Snippet: .. The gshB fragment was cloned at XbaI site of pUC19 (New England Biolabs) using in-fusion technique (In-Fusion HD cloning Kit, Clontech). ..

    Electroporation:

    Article Title: Supporting Information for FlaG competes with FliS-flagellin complexes for access to FlhA in the flagellar T3SS to control Campylobacter jejuni filament length
    Article Snippet: .. These fragments were joined together and inserted into the XbaI site of pEX18Gm by Gibson Assembly Master mix (New England Biolabs) to create pDARH5825. pDRH5825 was introduced by electroporation into PA14 to delete flaG from the chromosome based on previously described procedures (8, 9). ..



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